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Best Bet PCR methods and applications
A journal in our collection
Articles
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Acute‐onset smell and taste disorders in the context of COVID‐19: a pilot multicentre polymerase... 2020
https://ncsu.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwjV1La9wwEB7ygJJLyKO0btIwCTn0YrJ-aG2FEAjphuSQxyHt1ciWxC5svEvtPeSWnxAo_YP5JZ3Rep2aUMjFGCzZsj7NzCdp_BngUJMD1EKlfq-nebVKJ74sZO4XMQ2XXOQmUP3uYs7rr77m-hDtghtbhvPXbOAqrxZGfkSOgIycYu8yrAYsksaizvFd64R5e9HNtUTg87BrRIU6Vbuh6A2_fJsm-S99dfHnYgPWG-KIZ3OkN2HJlFvw4brZGt-GP2fFrDYvT8-cHl1j9WDGY1SlxloRkKgblc0KRyUS6UPOUSfHjBOL57c_r75TzUAeo8LpaDyp0SUauiYZPBmeTifjR16-qqjiUNEtiGu6LyJOjoanyKFQY0HHl6ffTfI7OuHaj_DjYnB_fuk3_1wgdEQa-aFKtLCCus-GOhLWaor3KtaGulhrQlUJWRCl6IuIyESRxzq12hBR44MxJvoMKBOZ5DZMrYplbIVVdB8hkqhfxEZZGXlwsOj3bDrX1MgWcxECJ3PgeLC7QCRrzKrKQiJ3ggKqlB7st5fJIHiXQ5VmMqMykWTWkcaBB5_mALZPiYg_9oiSepB0oG0LsNh290o5GjrR7YSYI72EB9_cIPh_w7PBzcCdfHl_0R1YC3ke73LXdmGl_jUzX2GFv-LYc-P6L-OuAME
European Journal Of Neurology, Vol. 27, Issue 9, pp. 1738 - 1741.
Background and purpose Specific respiratory tract infections, including COVID‐19, may cause smell and/or taste disorders (STDs) with increased frequency. The aim was to determine whether new‐onset ... Read more
Background and purpose Specific respiratory tract infections, including COVID‐19, may cause smell and/or taste disorders (STDs) with increased frequency. The aim was to determine whether new‐onset STDs are more frequent amongst COVID‐19 patients than influenza patients. Method This was a case–control study including hospitalized patients of two tertiary care centres. Consecutive patients positive for COVID‐19 polymerase chain reaction (cases) and patients positive for influenza polymerase chain reaction (historical control sample) were assessed during specific periods, employing a self‐reported STD questionnaire. Results Seventy‐nine cases and 40 controls were included. No significant differences were found in basal features between the two groups. New‐onset STDs were significantly more frequent amongst cases (31, 39.2%) than in the control group (5, 12.5 %) [adjusted odds ratio 21.4 (2.77–165.4, P = 0.003)]. COVID‐19 patients with new‐onset STDs were significantly younger than COVID‐19 patients without STDs (52.6 ± 17.2 vs. 67.4 ± 15.1, P < 0.001). Amongst COVID‐19 patients who presented STDs, 22 (70.9%) recalled an acute onset and it was an initial manifestation in 11 (35.5%). Twenty‐five (80.6%) presented smell disorders (mostly anosmia, 14, 45.2%) and 28 (90.3%) taste disorders (mostly ageusia, 14, 45.2%). Only four (12.9 %) reported concomitant nasal obstruction. The mean duration of STD was 7.5 ± 3.2 days and 12 patients (40%) manifested complete recovery after 7.4 ± 2.3 days of onset. Conclusion New‐onset STDs were significantly more frequent amongst COVID‐19 patients than influenza patients; they usually had an acute onset and were commonly an initial manifestation. The use of STD assessment in anamnesis as a hint for COVID‐19 and to support individuals’ self‐isolation in the current epidemic context is suggested. Read less
Journal Article | Full Text Online
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Diagnostic Accuracy of Novel and Traditional Rapid Tests for Influenza Infection Compared With... 2017
https://ncsu.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1ta9RAEF60gvhFfEPPVhnBr-Gued2VUjjOFpVelesV_RY2yYY7Knflelesv80_4r9xZl-ySS2i-CWEJLcJPM_NzM7OPsPY6woNYJVIHgwGFWWrqiwQpSiCMka6FEmhdmV6LZnz0-2FubGKvf_f0OI1BJe2yv4DvM2geAHPEWQ8Isx4_Cug35piORJeHZblZkX92ykNsLxURgYA3VE1tym_iTyf4xV0BVqHAc2D7lHyXdKZMm3DR64s_TPlaCeKCjeUUUHXJgb9HpXNXVE6C09HMzmnZQCzQ8LsdD_x-tBWuV_Xc6i1DKRVQWlHxV7FeW4SlF9_W_Mfq1V59s3k--Vi4Z2JNPu7J6Q47leVZs0Wx_ee_iN5JU2JAQUEsp3wQCdKDRu8WW1WslqZlU8uC3TRsfURks_-UjnzLtCkmobHjf03_UAs0dvWPDL9l697mSzSfTHH9Gnc6IR2hbyPP-aHp0dH-fTgy_Q2uxNmQlBzjZMPx02QEPGQu2aO9JFGF5lG7rtxu5HSTdOf0IZB0wfsvp2_wNDw8CG7pRaP2N2xResx--HpCI6OsKxB0xGQBdCiI2g6gqYjIB2hoSM0dARHRyA6gqUjdOgIe7N9z0jQjATHyL3-bP8NDMFzEgwn9dd0OPmEnR4eTEfvAtshJCjDQbgOcDZeUgiWoR3CefxuUSlRxipOKcyXpMZYZDIpZZGpNOV1Utd1Vpcc_Ropz8VV9IyByERW1CGvZSxifERGSY13oxQHkrWIeuyVgyE_NwowOc6cCaococoJqh7bcfjk1jRc5OjbMPwnvUccormN1puW5ORCLTf0DOkP4ixk0GNPDa7NS0LOUyF4-vzPg2-ze_5fssO21quNesG2yHa-1Jz7Bcn2yJg
Annals Of Internal Medicine, Vol. 167, Issue 6, pp. 394 - 409.
Rapid and accurate influenza diagnostics can improve patient care. To summarize and compare accuracy of traditional rapid influenza diagnostic tests (RIDTs), digital immunoassays (DIAs), and rapid ... Read more
Rapid and accurate influenza diagnostics can improve patient care. To summarize and compare accuracy of traditional rapid influenza diagnostic tests (RIDTs), digital immunoassays (DIAs), and rapid nucleic acid amplification tests (NAATs) in children and adults with suspected influenza. 6 databases from their inception through May 2017. Studies in English, French, or Spanish comparing commercialized rapid tests (that is, providing results in <30 minutes) with reverse transcriptase polymerase chain reaction reference standard for influenza diagnosis. Data were extracted using a standardized form; quality was assessed using QUADAS-2 (Quality Assessment of Diagnostic Accuracy Studies 2) criteria. 162 studies were included (130 of RIDTs, 19 of DIAs, and 13 of NAATs). Pooled sensitivities for detecting influenza A from Bayesian bivariate random-effects models were 54.4% (95% credible interval [CrI], 48.9% to 59.8%) for RIDTs, 80.0% (CrI, 73.4% to 85.6%) for DIAs, and 91.6% (CrI, 84.9% to 95.9%) for NAATs. Those for detecting influenza B were 53.2% (CrI, 41.7% to 64.4%) for RIDTs, 76.8% (CrI, 65.4% to 85.4%) for DIAs, and 95.4% (CrI, 87.3% to 98.7%) for NAATs. Pooled specificities were uniformly high (>98%). Forty-six influenza A and 24 influenza B studies presented pediatric-specific data; 35 influenza A and 16 influenza B studies presented adult-specific data. Pooled sensitivities were higher in children by 12.1 to 31.8 percentage points, except for influenza A by rapid NAATs (2.7 percentage points). Pooled sensitivities favored industry-sponsored studies by 6.2 to 34.0 percentage points. Incomplete reporting frequently led to unclear risk of bias. Underreporting of clinical variables limited exploration of heterogeneity. Few NAAT studies reported adult-specific data, and none evaluated point-of-care testing. Many studies had unclear risk of bias. Novel DIAs and rapid NAATs had markedly higher sensitivities for influenza A and B in both children and adults than did traditional RIDTs, with equally high specificities. Québec Health Research Fund and BD Diagnostic Systems. Read less
Journal Article | Full Text Online
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Digital polymerase chain reaction technology - recent advances and future perspectives 2018
https://ncsu.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwjV1LS8QwEB5EEPXgY33VFxG9lq2bpElABF0VQQ8e9FySNHWFZV3c9eDN_-A_9Jc4SR_uIoo9tikJmenM15nJNwBHORrAnGsZJ0nuo1W5iJVVJrYM1cVw4451Oh3MgaNfMvhUtbvytutxTHLuDS06LJ-RTW-aVAFn5Xk3JnxhkBI1B-nUq9Ne5weURMfyEhp-0MqxXC3DXX08J9ST-C4Gk4X17UBmGBgsJogb_1z-CixVIJOclVqxCjNu0IK5su3kWwvmu3WXtxYsThASrkF28fTou4iQk97p8Ln_5iNWI0dsTz8NCMLLcAjipN07JeMmJE8-3z_wma_yJFVFwYjoQU5KuhIy_D7OOVqHh6vL--51XLVgiC0CxXHsqLTC2FR2TIpAgpnCU9xJJ4xG6HecWK1xXC65KnjRKaxjhluTaMV5qtEY0i0gSihhio4sNFMMh2nKC84FTS1zulA0gsNaNtmwpNjIQmqcqux79yLYrcWWVZ_ZKOvg_10i8EojOGge4w76rIceuOdXP4YiKvaLjWCzFHczDUUT5gnrI9hA-Te3rezbMKvZ_tfadmABcZQMVS5sF2bHL69uD2a9ruwHZf0CydnjHw
Lab On A Chip, Vol. 18, Issue 24, pp. 3717 - 3732.
Digital polymerase chain reaction (dPCR) technology has remained a "hot topic" in the last two decades due to its potential applications in cell biology, genetic engineering, and medical diagnostic... Read more
Digital polymerase chain reaction (dPCR) technology has remained a "hot topic" in the last two decades due to its potential applications in cell biology, genetic engineering, and medical diagnostics. Various advanced techniques have been reported on sample dispersion, thermal cycling and output monitoring of digital PCR. However, a fully automated, low-cost and handheld digital PCR platform has not been reported in the literature. This paper attempts to critically evaluate the recent developments in techniques for sample dispersion, thermal cycling and output evaluation for dPCR. The techniques are discussed in terms of hardware simplicity, portability, cost-effectiveness and suitability for automation. The present paper also discusses the research gaps observed in each step of dPCR and concludes with possible improvements toward portable, low-cost and automatic digital PCR systems. We critically evaluate the state of the art of the development of digital polymerase chain reaction systems. Read less
Journal Article | Full Text Online
Books & Media
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Polymerase chain reaction
https://catalog.lib.ncsu.edu/catalog/NCSU738450
Hill QP606 .D46 P65 1989 | Book
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Polymerase chain reaction : theory and technology
https://catalog.lib.ncsu.edu/catalog/NCSU5875885
Online Resources QP606 .D46 .B445 2019 ebook | Book
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The Polymerase chain reaction
https://catalog.lib.ncsu.edu/catalog/NCSU836927
Hill QP606 .D46 P652 1994 | Book
Other Ways to Find Articles
Databases
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Current chemical reactions (CCR-Expanded)
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Descriptive Healthcare Analytics in R
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Learn how to conduct a full analysis of healthcare datasets using R by analyzing the Behavioral Risk Factor Surveillance System (BRFSS), a public health surveillance survey.
Learn how to conduct a full analysis of healthcare datasets using R by analyzing the Behavioral Risk Factor Surveillance System (BRFSS), a public health surveillance survey. Read less
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College of Agriculture & Life Sciences
https://www.lib.ncsu.edu/archivedexhibits/patents/CALS.htm
5,780,271 Click to enlarge image Dr. Jean Beagle Ristaino is a professor in the Department of Plant Pathology at NC State University. Her patent, "PCR [Polymerase Chain
5,780,271 Click to enlarge image Dr. Jean Beagle Ristaino is a professor in the Department of Plant Pathology at NC State University. Her patent, "PCR [Polymerase Chain Read less
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Charles W. Suggs Papers, 1914-1995
https://www.lib.ncsu.edu/findingaids/mc00033
10, Folder 25 Digital content Vibration Responses, Chain Saw and Tractor Cab (Envelope 33689) 11 Mar. 1968 Box 10, Folder 26 Digital content Vibration Simulator and Active
10, Folder 25 Digital content Vibration Responses, Chain Saw and Tractor Cab (Envelope 33689) 11 Mar. 1968 Box 10, Folder 26 Digital content Vibration Simulator and Active Read less
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NC State University Libraries - Textbook Service
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Conditions Instructors: Addonizio BUS 370 : Operations and Supply Chain Management Instructors: Brosnan, Favre FS 201 : Introduction to Food Science Instructors: Luck BCH 351
Conditions Instructors: Addonizio BUS 370 : Operations and Supply Chain Management Instructors: Brosnan, Favre FS 201 : Introduction to Food Science Instructors: Luck BCH 351 Read less