Kinetic Analysis of the Amino Terminal End of Active Site Loop of Lactate Deyhdrogenase from Plasmodium Vivax
Academic Journal
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Balkan Medical Journal, Vol 29, Iss 4, Pp 364-369 (2012)
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Dilek Turgut Balık;Özal Mutlu
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Balkan Medical Journal, Vol 29, Iss 4, Pp 364-369 (2012)
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Objective: In this study, kinetic analysis was performed to understand the functional ...
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Kinetic Analysis of the Amino Terminal End of Active Site Loop of Lactate Deyhdrogenase from Plasmodium Vivax
Balkan Medical Journal, Vol 29, Iss 4, Pp 364-369 (2012)
Objective: In this study, kinetic analysis was performed to understand the functional importance of the amino terminal of the active site of previously mutated Plasmodium vivax Lactate Dehydrogenase enzyme by mimicking Toxoplasma gondii I, II, Eimeria acervulina and Eimeria tenella LDH’s.Material and Methods: Mutant LDH genes were amplified by PCR and 6xHistag was added to the C-terminal of the enzymes. Then LDH enzymes are overproduced as recombinant in E. coli cells, purified by Ni-NTA agarose matrix and kinetic properties were analysed. Results: Observing increase of Km values of mutant enzymes showed that mutations in this place caused decreasing affinity of enzyme for its substrate. However kcat values were about the same throughout all mutant proteins. Conclusion: Sensitivity of the studied region emphasizes the significance of this site for drug design studies for both Plasmodium and some other Apicomplexans.
Subject terms:
Lactate dehydrogenase - Plasmodium vivax - antimalarial drug - site-directed mutagenesis - kinetic analysis - MedicineContent provider:
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"GELECEĞE BAKIŞ VE DEĞİŞİM". (Turkish)
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Conservative Thought / Muhafazakar Düşünce; eyl-ara2018, Vol. 15 Issue 55, p11-38, 28p
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ÖZAL, TURGUT
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Conservative Thought / Muhafazakar Düşünce; eyl-ara2018, Vol. 15 Issue 55, p11-38, 28p
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Preoperatif Nötrofil/Lenfosit Oranının Testis Tümörlerinin Evresinin Tespitindeki Prediktif Değeri. (Turkish)
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Journal of Turgut Ozal Medical Center; 2013, Vol. 20 Issue 3, p243-245, 3p
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Yıldırım, Mehmet Erol;Badem, Hüseyin;Karataş, Ömer Faruk;Çimentepe, Ersin;Ü...
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Journal of Turgut Ozal Medical Center; 2013, Vol. 20 Issue 3, p243-245, 3p
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Preoperatif Nötrofil/Lenfosit Oranının Testis Tümörlerinin Evresinin Tespitindeki Prediktif Değeri. (Turkish)
Journal of Turgut Ozal Medical Center; 2013, Vol. 20 Issue 3, p243-245, 3p
Copyright of Journal of Turgut Ozal Medical Center is the property of Annals of Medical Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Subject terms:
PREOPERATIVE care - PREDICTIVE validity - LYMPHOCYTES - LYMPHOMAS - CANCER patientsContent provider:
Complementary Index
A Programme to Make Capitalism Work.
Özal, Turgut
The article presents an interview with Turkey's Deputy Prime Minister, Turgut Özal. Tu...
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A Programme to Make Capitalism Work.
Euromoney. Feb1982 Supplement, p5-10. 6p.
The article presents an interview with Turkey's Deputy Prime Minister, Turgut Özal . Turkey's present economic policy dates from the day when Turgut Özal introduced the Economic Stabilization Programme, with its emphasis on exports and a realistic exchange rate. Turkey's present good relations with the International Monetary Fund are entirely due to the policy outlined by Turgut , on January 24, 1980. Regarding economic measures taken by his government, he says that tax holidays for exporters, allocations of foreign exchange for them, investment allowances in specific industries and regions, have been introduced. Customs duties are waived or paid in installments; funds are provided as seed money for private entrepreneurs. The government's major revolution is to bring in these incentives as a law, passed after the second five-year plan. It is called the Plan Implementation Law. But, for political reasons, some of the articles are ruled to be unconstitutional. Nevertheless, the government found a way round the problem. This was the decree which it has introduced after so much of the bill had been declared unconstitutional. In theory this is against the rules, but no one has ever tried to abolish it and it stands today. The government is still implementing the same decree it issued in 1969.
Subject terms:
ECONOMIC policy - ECONOMIC reform - GOVERNMENT policy - TURKEY - OZAL, Turgut, 1927-1993Content provider:
Business Source Premier
Kinetic Analysis of the Amino Terminal End of Active Site Loop of Lactate Deyhdrogenase from Plasmodium Vivax.
Academic Journal
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Balkan Medical Journal; Dec2012, Vol. 29 Issue 4, p364-369, 6p, 3 Diagrams, 2 Charts, 2 Graphs
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Mutlu, Özal;Balık, Dilek Turgut
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Balkan Medical Journal; Dec2012, Vol. 29 Issue 4, p364-369, 6p, 3 Diagrams, 2 Charts, 2 Graphs
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Objective: In this study, kinetic analysis was performed to understand the functional ...
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Kinetic Analysis of the Amino Terminal End of Active Site Loop of Lactate Deyhdrogenase from Plasmodium Vivax.
Balkan Medical Journal; Dec2012, Vol. 29 Issue 4, p364-369, 6p, 3 Diagrams, 2 Charts, 2 Graphs
Objective: In this study, kinetic analysis was performed to understand the functional importance of the amino terminal of the active site of previously mutated Plasmodium vivax Lactate Dehydrogenase enzyme by mimicking Toxoplasma gondii I, II, Eimeria acervulina and Eimeria tenella LDH's. Material and Methods: Mutant LDH genes were amplified by PCR and 6xHistag was added to the C-terminal of the enzymes. Then LDH enzymes are overproduced as recombinant in E. coli cells, purified by Ni-NTA agarose matrix and kinetic properties were analysed. Results: Observing increase of Km values of mutant enzymes showed that mutations in this place caused decreasing affinity of enzyme for its substrate. However kcat values were about the same throughout all mutant proteins. Conclusion: Sensitivity of the studied region emphasizes the significance of this site for drug design studies for both Plasmodium and some other Apicomplexans. [ABSTRACT FROM AUTHOR]
Subject terms:
TURKEY - ANTIMALARIALS - DYNAMICS - GENES - LACTATE dehydrogenase - POLYMERASE chain reaction - PROTOZOAN diseases - RESEARCH funding - WESTERN immunoblottingContent provider:
Complementary Index
SUPERİOR MEZENTERİK ARTER OKLÜZYONU VE MEZENTEROİLİAK BYPASS İLE TEDAVİSİ: OLGU SUNUMU.
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Anatolian Journal of Clinical Investigation. 2009, Vol. 3 Issue 2, p148-150. 3p. 2 Color Photographs, 2 Black and White Photographs.
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Özer, Mustafa Tahir;Uzar, Ali İhsan;Özal, Ertuğrul;Menteş, Öner;Biingöl, Ha...
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Anatolian Journal of Clinical Investigation. 2009, Vol. 3 Issue 2, p148-150. 3p. 2 Color Photographs, 2 Black and White Photographs.
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Mesenteric ischemia due to arterial occlusion is still an important cause of mortality...
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SUPERİOR MEZENTERİK ARTER OKLÜZYONU VE MEZENTEROİLİAK BYPASS İLE TEDAVİSİ: OLGU SUNUMU.
Anatolian Journal of Clinical Investigation. 2009, Vol. 3 Issue 2, p148-150. 3p. 2 Color Photographs, 2 Black and White Photographs.
Mesenteric ischemia due to arterial occlusion is still an important cause of mortality and morbidity. The goal of medical and surgical therapies is to restore decreased mesenteric circulation for the purpose of preventing intestinal ischemia or necrosis, malnutrition, weight loss and postprandial abdominal pain. Anticoagulant agents, percutaneous transluminal angioplasty, balloon / stent applications, and bypass surgery are preferred treatment alternatives. A successful iliaco- mesenteric bypass performed for a chronic superior mesenteric artery ischemia and it's 24 month follow up is presented in this case report in view of current literature. [ABSTRACT FROM AUTHOR]
Subject terms:
MESENTERIC artery - ABDOMINAL pain - PLASTIC surgery - NUTRITION disordersContent provider:
Academic Search Complete
Can serum interleukin 34 levels be used as an indicator for the prediction and prognosis of COVID-19?
Academic Journal
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Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
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Karahan D;Bolayir HA;Bolayir A;Demir B;Otlu Ö;Erdem M
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Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
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Objective: Interleukin 34 (IL-34) is a molecule whose expression is increased in condi...
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Can serum interleukin 34 levels be used as an indicator for the prediction and prognosis of COVID-19?
Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
Objective: Interleukin 34 (IL-34) is a molecule whose expression is increased in conditions such as autoimmune disorders, inflammation, and infections. Our study aims to determine the role of IL-34 in the diagnosis, follow-up, and prognosis of Coronavirus Disease-19 (COVID-19).
Method: A total of 80 cases were included in the study as 40 COVID-19 positive patient groups and 40 COVID-19 negative control groups. The COVID-19-positive group consisted of 20 intensive-care unit (ICU) patients and 20 outpatients. Serum IL-34, c-reactive protein (CRP), ferritin, D-dimer, troponin I, hemogram, and biochemical parameters of the cases were studied and compared between groups.
Results: IL-34 levels were significantly higher in the COVID-19-positive group than in the negative group. IL-34 levels increased in correlation with CRP in predicting the diagnosis of COVID-19. IL-34 levels higher than 31.75 pg/m predicted a diagnosis of COVID-19. IL-34 levels did not differ between the outpatient and ICU groups in COVID-19-positive patients. IL-34 levels were also not different between those with and without lung involvement.
Conclusion: While IL-34 levels increased in COVID-19-positive patients and were successful in predicting the diagnosis of COVID-19, it was not found to be significant in determining lung involvement, risk of intensive care hospitalization, and prognosis. The role of IL-34 in COVID-19 deserves further evaluation.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2024 Karahan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Method: A total of 80 cases were included in the study as 40 COVID-19 positive patient groups and 40 COVID-19 negative control groups. The COVID-19-positive group consisted of 20 intensive-care unit (ICU) patients and 20 outpatients. Serum IL-34, c-reactive protein (CRP), ferritin, D-dimer, troponin I, hemogram, and biochemical parameters of the cases were studied and compared between groups.
Results: IL-34 levels were significantly higher in the COVID-19-positive group than in the negative group. IL-34 levels increased in correlation with CRP in predicting the diagnosis of COVID-19. IL-34 levels higher than 31.75 pg/m predicted a diagnosis of COVID-19. IL-34 levels did not differ between the outpatient and ICU groups in COVID-19-positive patients. IL-34 levels were also not different between those with and without lung involvement.
Conclusion: While IL-34 levels increased in COVID-19-positive patients and were successful in predicting the diagnosis of COVID-19, it was not found to be significant in determining lung involvement, risk of intensive care hospitalization, and prognosis. The role of IL-34 in COVID-19 deserves further evaluation.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2024 Karahan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Subject terms:
Humans - SARS-CoV-2 metabolism - Retrospective Studies - Prognosis - C-Reactive Protein metabolism - Interleukins - COVID-19 diagnosisContent provider:
MEDLINE
The effect of 10 weeks of karate training on the development of motor skills in children who are new to karate.
Academic Journal
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Publisher: Frontiers Research Foundation Country of Publication: Switzerland NLM ID: 101549006 Publication Model: eCollection Cited Medium: Print ISSN: 1664-042X (Print) Linking ISSN: 1664042X NLM ISO Abbreviation: Front Physiol Subsets: PubMed not MEDLINE
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Arslan Y;Yavaşoğlu B;Beykumül A;Pekel AÖ;Suveren C;Karabulut EO;Ayyıldız Du...
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Publisher: Frontiers Research Foundation Country of Publication: Switzerland NLM ID: 101549006 Publication Model: eCollection Cited Medium: Print ISSN: 1664-042X (Print) Linking ISSN: 1664042X NLM ISO Abbreviation: Front Physiol Subsets: PubMed not MEDLINE
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This study investigated the effect of a 10-week karate training program on the motor s...
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The effect of 10 weeks of karate training on the development of motor skills in children who are new to karate.
Publisher: Frontiers Research Foundation Country of Publication: Switzerland NLM ID: 101549006 Publication Model: eCollection Cited Medium: Print ISSN: 1664-042X (Print) Linking ISSN: 1664042X NLM ISO Abbreviation: Front Physiol Subsets: PubMed not MEDLINE
This study investigated the effect of a 10-week karate training program on the motor skill development of 5-7-year-old children new to karate with two different test batteries. A total of 28 participants were included in the study: 18 in the Karate group and 10 in the control group. The karate group was subjected to a fundamental karate training (kihon) program consisting of 90-minute sessions four days a week for ten weeks in addition to physical education classes at their schools. In contrast, the control group did not participate in any sportive activities except physical education classes in their schools. Data were collected using the Eurofit test battery and the TGMD-2 test. In the pre-post test comparison of the anthropometric measurements of the karate group, no significant difference was found in the control group. In contrast, a significant difference was obtained in height, body mass index, and body fat percentage. In the post-test analysis of the two independent groups, there were statistically significant differences in favor of the karate group regarding height and body fat percentage ( p < 0.005). In the pre-post analysis of the Eurofit test and the TGMD-2 for the karate group, all parameters showed statistically significant improvements ( p < 0.001), while the control group showed no statistical difference. After comparing the karate and control groups, the Eurofit Test and TGMD-2 post-test results showed significantly higher scores (statistically significant differences) in all parameters for the karate group. In conclusion, the study shows that the 10-week karate training program positively affected the motor development of the participating children.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2024 Arslan, Yavaşoğlu, Beykumül, Pekel, Suveren, Karabulut, Ayyıldız Durhan, Çakır, Sarıakçalı, Küçük and Ceylan.)
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2024 Arslan, Yavaşoğlu, Beykumül, Pekel, Suveren, Karabulut, Ayyıldız Durhan, Çakır, Sarıakçalı, Küçük and Ceylan.)
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MEDLINE
Evaluation of hepatosteatosis and gallstone disease in patients with chronic kidney disease.
Academic Journal
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Publisher: Blackwell Pub Country of Publication: Canada NLM ID: 101093910 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1542-4758 (Electronic) Linking ISSN: 14927535 NLM ISO Abbreviation: Hemodial Int Subsets: MEDLINE
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Karahan D;Şahin İ
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Publisher: Blackwell Pub Country of Publication: Canada NLM ID: 101093910 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1542-4758 (Electronic) Linking ISSN: 14927535 NLM ISO Abbreviation: Hemodial Int Subsets: MEDLINE
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Introduction: The prevalence rates of hepatosteatosis and gallstones are increasing ow...
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Evaluation of hepatosteatosis and gallstone disease in patients with chronic kidney disease.
Publisher: Blackwell Pub Country of Publication: Canada NLM ID: 101093910 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1542-4758 (Electronic) Linking ISSN: 14927535 NLM ISO Abbreviation: Hemodial Int Subsets: MEDLINE
Introduction: The prevalence rates of hepatosteatosis and gallstones are increasing owing to the multifactorial causes of chronic kidney disease, and the prevalence may change with the availability of different forms of renal replacement therapy. We aimed to determine the incidence or prevalence rates of hepatosteatosis, cholelithiasis, and acute cholecystitis in patients with chronic kidney disease and compare them between renal replacement therapy modalities.
Methods: A total of 270 patients (90 with chronic kidney disease stages III-V, 90 undergoing peritoneal dialysis, and 90 undergoing hemodialysis) were included and categorized into the pre-dialysis, hemodialysis, and peritoneal dialysis groups. The patients were questioned about previous gallbladder surgeries and chronic diseases. The results of abdominal ultrasonography, tomography, and magnetic resonance imaging were retrospectively evaluated with respect to the findings on the hepatobiliary system. Hepatosteatosis and cholelithiasis were diagnosed by expert radiologists on the basis of abdominal ultrasonography, tomography, and magnetic resonance imaging findings. The prevalence rates of hepatosteatosis, cholelithiasis, and other liver findings were compared between the groups.
Findings: Hepatosteatosis and cholelithiasis were detected in 16.7% and 21.5% of the 270 cases, respectively. Hepatosteatosis was present in 17.8%, 25.6%, and 6.7% of patients in the pre-dialysis, hemodialysis, and peritoneal dialysis groups, respectively. The prevalence of hepatosteatosis was significantly higher in patients undergoing hemodialysis than in patients undergoing peritoneal dialysis (p = 0.002). However, no statistically significant difference was found between the peritoneal dialysis and pre-dialysis groups or between the hemodialysis and pre-dialysis groups (p >0.05). The prevalence rates of cholelithiasis were 15.6%, 28.9%, and 20.0%, in the pre-dialysis, hemodialysis, and peritoneal dialysis groups, respectively, and there were no statistically significant differences among the groups. The incidence of acute cholecystitis was significantly higher in the hemodialysis group than in the pre-dialysis group (p = 0.006).
Discussion: Our study showed that the hepatobiliary system is frequently affected in chronic kidney disease and that the findings may differ depending on the renal replacement therapy modality.
(© 2024 International Society for Hemodialysis.)
Methods: A total of 270 patients (90 with chronic kidney disease stages III-V, 90 undergoing peritoneal dialysis, and 90 undergoing hemodialysis) were included and categorized into the pre-dialysis, hemodialysis, and peritoneal dialysis groups. The patients were questioned about previous gallbladder surgeries and chronic diseases. The results of abdominal ultrasonography, tomography, and magnetic resonance imaging were retrospectively evaluated with respect to the findings on the hepatobiliary system. Hepatosteatosis and cholelithiasis were diagnosed by expert radiologists on the basis of abdominal ultrasonography, tomography, and magnetic resonance imaging findings. The prevalence rates of hepatosteatosis, cholelithiasis, and other liver findings were compared between the groups.
Findings: Hepatosteatosis and cholelithiasis were detected in 16.7% and 21.5% of the 270 cases, respectively. Hepatosteatosis was present in 17.8%, 25.6%, and 6.7% of patients in the pre-dialysis, hemodialysis, and peritoneal dialysis groups, respectively. The prevalence of hepatosteatosis was significantly higher in patients undergoing hemodialysis than in patients undergoing peritoneal dialysis (p = 0.002). However, no statistically significant difference was found between the peritoneal dialysis and pre-dialysis groups or between the hemodialysis and pre-dialysis groups (p >0.05). The prevalence rates of cholelithiasis were 15.6%, 28.9%, and 20.0%, in the pre-dialysis, hemodialysis, and peritoneal dialysis groups, respectively, and there were no statistically significant differences among the groups. The incidence of acute cholecystitis was significantly higher in the hemodialysis group than in the pre-dialysis group (p = 0.006).
Discussion: Our study showed that the hepatobiliary system is frequently affected in chronic kidney disease and that the findings may differ depending on the renal replacement therapy modality.
(© 2024 International Society for Hemodialysis.)
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MEDLINE
Evaluation of cardiac autonomic dysfunctions in children with type 1 diabetes mellitus.
Academic Journal
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Publisher: BioMed Central Country of Publication: England NLM ID: 100967804 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2431 (Electronic) Linking ISSN: 14712431 NLM ISO Abbreviation: BMC Pediatr Subsets: MEDLINE
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Gözüküçük D;İleri BA;Başkan SK;Öztarhan E;Güller D;Önal H;Öztarhan K
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Publisher: BioMed Central Country of Publication: England NLM ID: 100967804 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2431 (Electronic) Linking ISSN: 14712431 NLM ISO Abbreviation: BMC Pediatr Subsets: MEDLINE
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Background: Cardiovascular autonomic neuropathy (CAN) is a serious complication of dia...
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Evaluation of cardiac autonomic dysfunctions in children with type 1 diabetes mellitus.
Publisher: BioMed Central Country of Publication: England NLM ID: 100967804 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2431 (Electronic) Linking ISSN: 14712431 NLM ISO Abbreviation: BMC Pediatr Subsets: MEDLINE
Background: Cardiovascular autonomic neuropathy (CAN) is a serious complication of diabetes, impacting the autonomic nerves that regulate the heart and blood vessels. Timely recognition and treatment of CAN are crucial in averting the onset of cardiovascular complications. Both clinically apparent autonomic neuropathy and subclinical autonomic neuropathy, particularly CAN pose a significant risk of morbidity and mortality in children with type 1 diabetes mellitus (T1DM). Notably, CAN can progress silently before manifesting clinically. In our study, we assessed patients with poor metabolic control, without symptoms, following the ISPAD 2022 guideline. The objective is is to determine which parameters we can use to diagnose CAN in the subclinical period.
Methods: Our study is a cross-sectional case-control study that includes 30 children diagnosed with T1DM exhibiting poor metabolic control (average HbA1c > 8.5% for at least 1 year) according to the ISPAD 2022 Consensus Guide. These patients, who are under the care of the pediatric diabetes clinic, underwent evaluation through four noninvasive autonomic tests: echocardiography, 24-h Holter ECG for heart rate variability (HRV), cardiopulmonary exercise test, and tilt table test.
Results: The average age of the patients was 13.73 ± 1.96 years, the average diabetes duration was 8 ± 3.66 years, and the 1-year average HbA1c value was 11.34 ± 21%. In our asymptomatic and poorly metabolically controlled patient group, we found a decrease in HRV values, the presence of postural hypotension with the tilt table test, and a decrease in ventricular diastolic functions that are consistent with the presence of CAN. Despite CAN, the systolic functions of the ventricles were preserved, and the dimensions of the cardiac chambers and cardiopulmonary exercise test were normal.
Conclusions: CAN is a common complication of T1DM, often associated with the patient's age and poor glycemic control. HRV, active orthostatic tests, and the evaluation of diastolic dysfunctions play significant roles in the comprehensive assessment of CAN. These diagnostic measures are valuable tools in identifying autonomic dysfunction at an early stage, allowing for timely intervention and management to mitigate the impact of cardiovascular complications associated with T1DM.
(© 2024. The Author(s).)
Methods: Our study is a cross-sectional case-control study that includes 30 children diagnosed with T1DM exhibiting poor metabolic control (average HbA1c > 8.5% for at least 1 year) according to the ISPAD 2022 Consensus Guide. These patients, who are under the care of the pediatric diabetes clinic, underwent evaluation through four noninvasive autonomic tests: echocardiography, 24-h Holter ECG for heart rate variability (HRV), cardiopulmonary exercise test, and tilt table test.
Results: The average age of the patients was 13.73 ± 1.96 years, the average diabetes duration was 8 ± 3.66 years, and the 1-year average HbA1c value was 11.34 ± 21%. In our asymptomatic and poorly metabolically controlled patient group, we found a decrease in HRV values, the presence of postural hypotension with the tilt table test, and a decrease in ventricular diastolic functions that are consistent with the presence of CAN. Despite CAN, the systolic functions of the ventricles were preserved, and the dimensions of the cardiac chambers and cardiopulmonary exercise test were normal.
Conclusions: CAN is a common complication of T1DM, often associated with the patient's age and poor glycemic control. HRV, active orthostatic tests, and the evaluation of diastolic dysfunctions play significant roles in the comprehensive assessment of CAN. These diagnostic measures are valuable tools in identifying autonomic dysfunction at an early stage, allowing for timely intervention and management to mitigate the impact of cardiovascular complications associated with T1DM.
(© 2024. The Author(s).)
Subject terms:
Humans - Child - Adolescent - Cross-Sectional Studies - Case-Control Studies - Glycated Hemoglobin - Heart Rate physiology - Diabetes Mellitus, Type 1 complications - Diabetic Neuropathies diagnosis - Diabetic Neuropathies etiology - Autonomic Nervous System Diseases diagnosis - Autonomic Nervous System Diseases etiologyContent provider:
MEDLINE
Ameliorative Effects of Larazotide Acetate on Intestinal Permeability and Bacterial Translocation in Acute Pancreatitis Model in Rats.
Academic Journal
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Publisher: Springer Science + Business Media Country of Publication: United States NLM ID: 7902782 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1573-2568 (Electronic) Linking ISSN: 01632116 NLM ISO Abbreviation: Dig Dis Sci Subsets: In Process; MEDLINE
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Karahan D;Harputluoglu MMM;Gul M;Gunduz A;Ozyalin F;İnceoğlu F;Tikici D;Yıl...
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Publisher: Springer Science + Business Media Country of Publication: United States NLM ID: 7902782 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1573-2568 (Electronic) Linking ISSN: 01632116 NLM ISO Abbreviation: Dig Dis Sci Subsets: In Process; MEDLINE
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Background: Intestinal barrier dysfunction in acute pancreatitis (AP) may progress to ...
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Ameliorative Effects of Larazotide Acetate on Intestinal Permeability and Bacterial Translocation in Acute Pancreatitis Model in Rats.
Publisher: Springer Science + Business Media Country of Publication: United States NLM ID: 7902782 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1573-2568 (Electronic) Linking ISSN: 01632116 NLM ISO Abbreviation: Dig Dis Sci Subsets: In Process; MEDLINE
Background: Intestinal barrier dysfunction in acute pancreatitis (AP) may progress to systemic inflammatory response syndrome (SIRS) and multi-organ failures by causing bacterial translocation. Larazotide acetate (LA) is a molecule that acts as a tight junction (TJ) regulator by blocking zonulin (Zo) receptors in the intestine.
Aims: In our study, we aimed to investigate the effects of LA on intestinal barrier dysfunction and bacterial translocation in the AP model in rats.
Methods: Thirty-two male Sprague-Dawley rats were divided into 4 groups; control, larazotide (LAR), AP, and AP + LAR. The AP model was created by administering 250 mg/100 g bm L-Arginine intraperitoneally 2 times with an hour interval. AP + LAR group received prophylactic 0.01 mg/mL LA orally for 7 days before the first dose of L-Arginine. For intestinal permeability analysis, fluorescein isothiocyanate-dextran (FITC-Dextran) was applied to rats by gavage. The positivity of any of the liver, small intestine mesentery, and spleen cultures were defined as bacterial translocation. Histopathologically damage and zonulin immunoreactivity in the intestine were investigated.
Results: Compared to the control group, the intestinal damage scores, anti-Zo-1 immunoreactivity H-Score, serum FITC-Dextran levels and bacterial translocation frequency (100% versus 0%) in the AP group were significantly higher (all p < 0.01). Intestinal damage scores, anti-Zo-1 immunoreactivity H-score, serum FITC-Dextran levels, and bacterial translocation frequency (50% versus 100%) were significantly lower in the AP + LAR group compared to the AP group (all p < 0.01).
Conclusions: Our findings show that LA reduces the increased intestinal permeability and intestinal damage by its effect on Zo in the AP model in rats, and decreases the frequency of bacterial translocation as a result of these positive effects.
(© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Aims: In our study, we aimed to investigate the effects of LA on intestinal barrier dysfunction and bacterial translocation in the AP model in rats.
Methods: Thirty-two male Sprague-Dawley rats were divided into 4 groups; control, larazotide (LAR), AP, and AP + LAR. The AP model was created by administering 250 mg/100 g bm L-Arginine intraperitoneally 2 times with an hour interval. AP + LAR group received prophylactic 0.01 mg/mL LA orally for 7 days before the first dose of L-Arginine. For intestinal permeability analysis, fluorescein isothiocyanate-dextran (FITC-Dextran) was applied to rats by gavage. The positivity of any of the liver, small intestine mesentery, and spleen cultures were defined as bacterial translocation. Histopathologically damage and zonulin immunoreactivity in the intestine were investigated.
Results: Compared to the control group, the intestinal damage scores, anti-Zo-1 immunoreactivity H-Score, serum FITC-Dextran levels and bacterial translocation frequency (100% versus 0%) in the AP group were significantly higher (all p < 0.01). Intestinal damage scores, anti-Zo-1 immunoreactivity H-score, serum FITC-Dextran levels, and bacterial translocation frequency (50% versus 100%) were significantly lower in the AP + LAR group compared to the AP group (all p < 0.01).
Conclusions: Our findings show that LA reduces the increased intestinal permeability and intestinal damage by its effect on Zo in the AP model in rats, and decreases the frequency of bacterial translocation as a result of these positive effects.
(© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
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MEDLINE
Ecological risk assessment, source identification and spatial distribution of organic contaminants in terms of mucilage threat in streams of Çanakkale Strait Basin (Türkiye).
Academic Journal
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Publisher: Elsevier Science Ltd Country of Publication: England NLM ID: 0320657 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-1298 (Electronic) Linking ISSN: 00456535 NLM ISO Abbreviation: Chemosphere Subsets: MEDLINE
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Tokatlı C;Varol M;Uğurluoğlu A
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Publisher: Elsevier Science Ltd Country of Publication: England NLM ID: 0320657 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-1298 (Electronic) Linking ISSN: 00456535 NLM ISO Abbreviation: Chemosphere Subsets: MEDLINE
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In this study, the spatial distributions of organic contamination stressors in water o...
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Ecological risk assessment, source identification and spatial distribution of organic contaminants in terms of mucilage threat in streams of Çanakkale Strait Basin (Türkiye).
Publisher: Elsevier Science Ltd Country of Publication: England NLM ID: 0320657 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-1298 (Electronic) Linking ISSN: 00456535 NLM ISO Abbreviation: Chemosphere Subsets: MEDLINE
In this study, the spatial distributions of organic contamination stressors in water of fluvial habitats in the Çanakkale Strait (ÇS) watershed were investigated and the data were assessed in terms of human health and mucilage threat. Seven significant riverine ecosystems flowing into the ÇS were defined in the basin. Water samples were taken in the spring season (2023), when the phytoplankton communities reach their highest densities. Then they were tested for a total of 8 limnological parameters. The Nutrient Pollution Index (NPI) and Water Quality Index (WQI) were applied to assess the comprehensive quality characteristics of waters. The Hazard Quotient (HQ) and Hazard Index (HI) were applied to indicate the prospective non-carcinogenic human health risks of organic stressors. Principal Component Analysis (PCA) and Cluster Analysis (CA) were applied to categorize the investigated habitats and define the sources of investigated contamination parameters. Also, Geographic Information System (GIS) was used to make an effective assessment through visualization. The determined spatial mean values of the measured variables in ÇS watershed as follows: 18.21 °C for temperature, 8.51 mg/L for DO, 4.57 NTU for turbidity, 3.95 mg/L for suspended solids, 1.11 mg/L for NO 3 -N, 0.012 mg/L for NO 2 -N; 0.173 mg/L for PO 4 -P and 2.32 mg/L for BOD. It has been determined that the organic pollution loads and water temperature values of the investigated sub-basins increase from the upstream to the downstream locations and Çanakkale Stream was recorded as the riskiest fluvial habitat for the ÇS watershed. According to the results of health risk assessment indices, non-carcinogenic risks of organic pollutants would not be expected for all age groups.
Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interestsCem Tokatli reports financial support was provided by Trakya University.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interestsCem Tokatli reports financial support was provided by Trakya University.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Subject terms:
Humans - Ecosystem - Prospective Studies - Turkey - Water Quality - Risk Assessment - Environmental Monitoring methods - Water Pollutants, Chemical analysisContent provider:
MEDLINE
Metals and phosphorus in the sediments of streams emptying into the Çanakkale strait (Dardanelles): Spatial distribution, pollution status, risk assessment and source identification.
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Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0147621 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-0953 (Electronic) Linking ISSN: 00139351 NLM ISO Abbreviation: Environ Res Subsets: MEDLINE
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Varol M;Tokatlı C
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Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0147621 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-0953 (Electronic) Linking ISSN: 00139351 NLM ISO Abbreviation: Environ Res Subsets: MEDLINE
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The Çanakkale Strait is exposed to various pollutants due to its strategic location. I...
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Metals and phosphorus in the sediments of streams emptying into the Çanakkale strait (Dardanelles): Spatial distribution, pollution status, risk assessment and source identification.
Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0147621 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-0953 (Electronic) Linking ISSN: 00139351 NLM ISO Abbreviation: Environ Res Subsets: MEDLINE
The Çanakkale Strait is exposed to various pollutants due to its strategic location. It is thought that stream inputs may contribute significantly to metal and phosphorus (P) accumulation in the strait. In this study, the spatial distribution, pollution status, ecological risks and possible sources of twelve metals and P in the sediments of seven important streams emptying into the strait were analyzed. The results showed that Zn (226 mg/kg), Ba (67.2 mg/kg) and Pb (10.4 mg/kg) concentrations were higher in the Umurbey Stream due to mining activities, while P concentration (295 mg/kg) was higher in the Çanakkale Stream due to both agricultural activities and domestic wastewater discharges. Modified hazard quotient (mHQ), enrichment factor (EF) and contamination factor (Cf) values revealed that Zn and Pb showed high and moderate contamination in the US3 and US4 sampling sites of the Umurbey Stream, respectively. Similarly, P showed moderate contamination in the ÇS3 site of the Çanakkale Stream. Nemerow pollution index (NPI) showed that the US3 (2.41) and US4 (4.28) sites of the Umurbey Stream were slightly and moderately polluted, respectively. Toxic risk index (TRI) values demonstrated that the sediments in only the US4 site (5.17) of the Umurbey Stream may pose a low toxic risk due to high Zn content. Similarly, based on comparison results with sediment quality guidelines (SQGs), it was found that high Zn content may lead to adverse effects on sediment-dwelling organisms in the US4 site. In addition, the PEC-quotient value in the US4 site exceeded 0.5, confirming the finding that the sediments in this site could be toxic to benthic organisms. Finally, correlation, cluster and factor analyzes were used to determine possible sources of elements. Mining activities, natural sources and mixed sources (agricultural activities and natural sources) were identified as the main sources of elements in the sediments of the streams. This study can provide an important reference for evaluating stream sediment pollution and managing marine pollution.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024. Published by Elsevier Inc.)
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024. Published by Elsevier Inc.)
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The Effects of Workload Excess on Quality of Work Life in Third-Level Healthcare Workers: A Structural Equation Modeling Perspective.
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Publisher: MDPI AG Country of Publication: Switzerland NLM ID: 101666525 Publication Model: Electronic Cited Medium: Print ISSN: 2227-9032 (Print) Linking ISSN: 22279032 NLM ISO Abbreviation: Healthcare (Basel) Subsets: PubMed not MEDLINE
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Koca M;Deniz S;İnceoğlu F;Kılıç A
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Publisher: MDPI AG Country of Publication: Switzerland NLM ID: 101666525 Publication Model: Electronic Cited Medium: Print ISSN: 2227-9032 (Print) Linking ISSN: 22279032 NLM ISO Abbreviation: Healthcare (Basel) Subsets: PubMed not MEDLINE
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Examining the workload and working conditions of healthcare workers is crucial regardi...
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The Effects of Workload Excess on Quality of Work Life in Third-Level Healthcare Workers: A Structural Equation Modeling Perspective.
Publisher: MDPI AG Country of Publication: Switzerland NLM ID: 101666525 Publication Model: Electronic Cited Medium: Print ISSN: 2227-9032 (Print) Linking ISSN: 22279032 NLM ISO Abbreviation: Healthcare (Basel) Subsets: PubMed not MEDLINE
Examining the workload and working conditions of healthcare workers is crucial regarding the quality of the work life of employees and the quality of services provided. This study aims to determine the effects of the perceptions of workload on the quality of work life of health personnel working in two different tertiary hospitals affiliated with the Ministry of Health and the Higher Education Institution in Türkiye with structural equation modeling. This study was conducted in two hospitals in Malatya province: İnönü University Turgut Özal Medical Center affiliated with the Higher Education Institution and Malatya Training and Research Hospital affiliated with the Ministry of Health. The mean score of the Individual Workload Perception Scale was 106.18 ± 16.5, and the mean score of the Work-related Quality of Life Scale was 66.39 ± 15.33. Total workload scores had a statistically significant positive effect on total quality of work life scores (β1 = 0.98; p = 0.001). It was concluded that there was a statistically significant relationship between workload and quality of work life and age, unit of employment, working time in the profession, and hospital type.
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Determination of chemical components of the endemic species Allium turcicum L. plant extract by LC-MS/MS and evaluation of medicinal potentials.
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Publisher: Elsevier Ltd Country of Publication: England NLM ID: 101672560 Publication Model: eCollection Cited Medium: Print ISSN: 2405-8440 (Print) Linking ISSN: 24058440 NLM ISO Abbreviation: Heliyon Subsets: PubMed not MEDLINE
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İpek P;Atalar MN;Baran A;Baran MF;Ommati MM;Karadag M;Zor M;Eftekhari A;Alm...
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Publisher: Elsevier Ltd Country of Publication: England NLM ID: 101672560 Publication Model: eCollection Cited Medium: Print ISSN: 2405-8440 (Print) Linking ISSN: 24058440 NLM ISO Abbreviation: Heliyon Subsets: PubMed not MEDLINE
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The Allium turcicum L. (Zuzubak) plant as a cultivated vegetable have various health b...
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Determination of chemical components of the endemic species Allium turcicum L. plant extract by LC-MS/MS and evaluation of medicinal potentials.
Publisher: Elsevier Ltd Country of Publication: England NLM ID: 101672560 Publication Model: eCollection Cited Medium: Print ISSN: 2405-8440 (Print) Linking ISSN: 24058440 NLM ISO Abbreviation: Heliyon Subsets: PubMed not MEDLINE
The Allium turcicum L. (Zuzubak) plant as a cultivated vegetable have various health benefits and consumed as a food. Due to the shortcoming evidence in literature and the importance of this plant in folk medicine, in the present study, for the first time, we evaluated the bioactive profile of components (using LC-MS/MS), cytotoxicity, anticancer, antioxidant, and antibacterial prospectives of Zuzubak methanol extract. Reported results show that the extract is rich in bioactive compounds and has anticancer activity with breast cancer cells (MCF-7), human prostate cancer cells (DU-145), and Human osteosarcoma cancer Cell lines of (IC50) in dose dependent manner in the concentration range of 31.25 μg/mL and 2000 μg/mL for 24 and 48 h. Western blotting results determined that the extract significantly suppressed the growth of U2OS, MCF-7, and DU-145 cancer cells by down expression of Ang-1 (angiogenic protein) and Beclin-1 (autophagy protein) and overexpression of Bax (a proapoptotic protein). The oxidative stress indices showed a reduction in RPE-1 and MCF-7 cells and an upsurge in U2OS and DU-145 cells. Additionally, the antimicrobial assay showed suppression of the growth of various pathogenic microorganisms in 4.00-8.00 μg/concentrations of Zuzubak extract using the microdilution method. The phytochemicals identified showed promising anticancer, antioxidant effects, and antimicrobial properties, representing a valuable herbal source for drug development studies.
Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(© 2024 The Authors.)
Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(© 2024 The Authors.)
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Evaluation of femoral condyle volumes in patients with trochlear dysplasia: a pilot study.
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Publisher: Blackwell Scientific Publications Ltd Country of Publication: England NLM ID: 1306016 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1365-229X (Electronic) Linking ISSN: 00099260 NLM ISO Abbreviation: Clin Radiol Subsets: MEDLINE
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Pence KB;Sen S;Ormeci T;Caliskan M;Sakul BU
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Publisher: Blackwell Scientific Publications Ltd Country of Publication: England NLM ID: 1306016 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1365-229X (Electronic) Linking ISSN: 00099260 NLM ISO Abbreviation: Clin Radiol Subsets: MEDLINE
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Aim: To examine the volumes of the medial and lateral femoral condyles and the trochle...
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Evaluation of femoral condyle volumes in patients with trochlear dysplasia: a pilot study.
Publisher: Blackwell Scientific Publications Ltd Country of Publication: England NLM ID: 1306016 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1365-229X (Electronic) Linking ISSN: 00099260 NLM ISO Abbreviation: Clin Radiol Subsets: MEDLINE
Aim: To examine the volumes of the medial and lateral femoral condyles and the trochlear groove, and to determine their relationship with trochlear dysplasia.
Materials and Methods: Computed tomography images of the knees of 21 patients with trochlear dysplasia and 18 healthy individuals were analysed retrospectively. According to the modified Dejour classification, the degree of trochlear dysplasia was recorded, such as type A trochlea being low-grade dysplasia and types B, C, and D trochlea being high-grade dysplasia. Volume of the trochlear groove and medial and lateral femoral condyles were calculated using the three-dimensional (3D) volume-rendering method.
Results: The volumes of lateral condyle, medial condyle, and trochlear groove were statistically significantly lower in patients with trochlear dysplasia when compared to healthy participants. When compared according to Dejour types, all parameters were statistically significantly lower in both high- and low-grade dysplasia patients (p<0.05). The correlation between femoral condyle volumes and trochlear groove volume was low in the control group, but there was a moderate positive correlation in the patient group (r=0.50-0.75, p<0.05).
Conclusion: In trochlear dysplasia, the volume of the trochlear groove decreases and hypoplasia may occur in both lateral femoral and medial femoral condyles. Therefore, hypoplasia of only one condyle should not be interpreted as trochlear dysplasia. A holistic approach to patients is required.
(Copyright © 2023. Published by Elsevier Ltd.)
Materials and Methods: Computed tomography images of the knees of 21 patients with trochlear dysplasia and 18 healthy individuals were analysed retrospectively. According to the modified Dejour classification, the degree of trochlear dysplasia was recorded, such as type A trochlea being low-grade dysplasia and types B, C, and D trochlea being high-grade dysplasia. Volume of the trochlear groove and medial and lateral femoral condyles were calculated using the three-dimensional (3D) volume-rendering method.
Results: The volumes of lateral condyle, medial condyle, and trochlear groove were statistically significantly lower in patients with trochlear dysplasia when compared to healthy participants. When compared according to Dejour types, all parameters were statistically significantly lower in both high- and low-grade dysplasia patients (p<0.05). The correlation between femoral condyle volumes and trochlear groove volume was low in the control group, but there was a moderate positive correlation in the patient group (r=0.50-0.75, p<0.05).
Conclusion: In trochlear dysplasia, the volume of the trochlear groove decreases and hypoplasia may occur in both lateral femoral and medial femoral condyles. Therefore, hypoplasia of only one condyle should not be interpreted as trochlear dysplasia. A holistic approach to patients is required.
(Copyright © 2023. Published by Elsevier Ltd.)
Subject terms:
Humans - Pilot Projects - Retrospective Studies - Knee Joint diagnostic imaging - Femur diagnostic imaging - Joint InstabilityContent provider:
MEDLINE
The effects of disease severity and comorbidity on oxidative stress biomarkers in obstructive sleep apnea.
Academic Journal
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Publisher: Springer Country of Publication: Germany NLM ID: 9804161 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1522-1709 (Electronic) Linking ISSN: 15209512 NLM ISO Abbreviation: Sleep Breath Subsets: MEDLINE
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Kıran TR;Otlu Ö;Erdem M;Geçkil AA;Berber NK;İn E
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Publisher: Springer Country of Publication: Germany NLM ID: 9804161 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1522-1709 (Electronic) Linking ISSN: 15209512 NLM ISO Abbreviation: Sleep Breath Subsets: MEDLINE
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Purpose: Ischemia-modified albumin (IMA), total oxidant status (TOS), and total antiox...
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The effects of disease severity and comorbidity on oxidative stress biomarkers in obstructive sleep apnea.
Publisher: Springer Country of Publication: Germany NLM ID: 9804161 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1522-1709 (Electronic) Linking ISSN: 15209512 NLM ISO Abbreviation: Sleep Breath Subsets: MEDLINE
Purpose: Ischemia-modified albumin (IMA), total oxidant status (TOS), and total antioxidant status (TAS) are biomarkers used to evaluate oxidative stress status in various diseases including obstructive sleep apnea (OSA). In this study, we investigated the effects of disease severity and comorbidity on IMA, TOS and TAS levels in OSA.
Methods: Patients with severe OSA (no-comorbidity, one comorbidity, and multiple comorbidities) and mild-moderate OSA (no-comorbidity, one and multiple comorbidities), and healthy controls were included in the study. Polysomnography was applied to all cases and blood samples were taken from each participant at the same time of day. ELISA was used to measure IMA levels in serum samples and colorimetric commercial kits were used to perform TOS and TAS analyses. In addition, routine biochemical analyses were performed on all serum samples.
Results: A total of 74 patients and 14 healthy controls were enrolled. There was no statistically significant difference between the disease groups according to gender, smoking status, age, body mass index (BMI), HDL, T3, T4, TSH, and B12 (p > 0.05). As the severity of OSA and comorbidities increased, IMA, TOS, apnea-hypopnea index (AHI), desaturation index (T90), cholesterol, LDL, triglyceride, AST, and CRP values increased significantly (p < 0.05). On the other hand, TAS, minimum desaturation, and mean desaturation values decreased significantly (p < 0.05).
Conclusions: We concluded that IMA, TOS, and TAS levels may indicate OSA-related oxidative stress, but as the severity of OSA increases and with the presence of comorbidity, IMA and TOS levels may increase and TAS levels decrease. These findings suggest that disease severity and presence/absence of comorbidity should be considered in studies on OSA.
(© 2023. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)
Methods: Patients with severe OSA (no-comorbidity, one comorbidity, and multiple comorbidities) and mild-moderate OSA (no-comorbidity, one and multiple comorbidities), and healthy controls were included in the study. Polysomnography was applied to all cases and blood samples were taken from each participant at the same time of day. ELISA was used to measure IMA levels in serum samples and colorimetric commercial kits were used to perform TOS and TAS analyses. In addition, routine biochemical analyses were performed on all serum samples.
Results: A total of 74 patients and 14 healthy controls were enrolled. There was no statistically significant difference between the disease groups according to gender, smoking status, age, body mass index (BMI), HDL, T3, T4, TSH, and B12 (p > 0.05). As the severity of OSA and comorbidities increased, IMA, TOS, apnea-hypopnea index (AHI), desaturation index (T90), cholesterol, LDL, triglyceride, AST, and CRP values increased significantly (p < 0.05). On the other hand, TAS, minimum desaturation, and mean desaturation values decreased significantly (p < 0.05).
Conclusions: We concluded that IMA, TOS, and TAS levels may indicate OSA-related oxidative stress, but as the severity of OSA increases and with the presence of comorbidity, IMA and TOS levels may increase and TAS levels decrease. These findings suggest that disease severity and presence/absence of comorbidity should be considered in studies on OSA.
(© 2023. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)
Subject terms:
Humans - Biomarkers - Oxidative Stress - Comorbidity - Antioxidants - Patient Acuity - Serum Albumin - Sleep Apnea, Obstructive diagnosis - Sleep Apnea, Obstructive epidemiologyContent provider:
MEDLINE
The role of International Society of Paediatric Oncology (SIOP) in advancing global childhood cancer care.
Academic Journal
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Publisher: ecancer Country of Publication: England NLM ID: 101392236 Publication Model: eCollection Cited Medium: Print ISSN: 1754-6605 (Print) Linking ISSN: 17546605 NLM ISO Abbreviation: Ecancermedicalscience Subsets: PubMed not MEDLINE
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Challinor J;Davidson A;Chantada G;Kebudi R;Pritchard-Jones K
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Publisher: ecancer Country of Publication: England NLM ID: 101392236 Publication Model: eCollection Cited Medium: Print ISSN: 1754-6605 (Print) Linking ISSN: 17546605 NLM ISO Abbreviation: Ecancermedicalscience Subsets: PubMed not MEDLINE
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The Société Internationale d'Oncologie Pédiatrique [International Society of Paediatri...
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The role of International Society of Paediatric Oncology (SIOP) in advancing global childhood cancer care.
Publisher: ecancer Country of Publication: England NLM ID: 101392236 Publication Model: eCollection Cited Medium: Print ISSN: 1754-6605 (Print) Linking ISSN: 17546605 NLM ISO Abbreviation: Ecancermedicalscience Subsets: PubMed not MEDLINE
The Société Internationale d'Oncologie Pédiatrique [International Society of Paediatric Oncology] (SIOP), founded in 1969, aims to improve the lives of children and adolescents with cancer through global collaboration, education, training, research and advocacy. The annual congress provides the opportunity to share late-breaking research, clinical experiences and debate, with experts worldwide. SIOP's six Continental Branches represent their constituent members in North America, Oceania, Latin America, Africa, Europe and Asia and bring best practices and recent research findings of value to their specific patient populations. In 1990, the SIOP Board of Directors addressed the formerly predominantly European/North American society transforming into a global association by establishing a scholarship program to bring low- and middle-income country (LMIC) paediatric oncologists and nurses to SIOP meetings. A major achievement was SIOP's acceptance as a World Health Organisation (WHO) non-state actor in official relations in 2018, joining 220 non-governmental organisations, international business associations and philanthropic foundations with this privilege. SIOP supports advocacy with WHO member states and civil society to highlight the specific needs of cancer in this age-group through key programs especially supporting the WHO Global Initiative for Childhood Cancer. Sustained improvement in childhood cancer outcomes has paralleled the integration of research with care; thus, SIOP launched a Programme for Advancing Research Capacity for funding selected clinical trial groups in LMICs. SIOP supports south-south partnerships, and the principles elegantly expressed in SIOP Africa's checklist for co-branding projects, that include the prioritisation of local needs, cultivation of local expertise and commitment to equitable partnerships. SIOP now counts approximately 3,000 members from over 128 countries; 39% are from more than 60 LMICs. SIOP members have multidisciplinary expertise on all aspects of childhood cancer care working in collaboration with key stakeholders including governments, civil society organisations and funders to improve the lives of children/adolescents with cancer everywhere in all ways.
Competing Interests: The authors declare no financial conflicts of interest.
(© the authors; licensee ecancermedicalscience.)
Competing Interests: The authors declare no financial conflicts of interest.
(© the authors; licensee ecancermedicalscience.)
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Recent outcomes of liver transplantation for Budd-Chiari syndrome: A study of the European Liver Transplant Registry (ELTR) and affiliated centers.
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Publisher: Wolters Kluwer Health, Inc Country of Publication: United States NLM ID: 8302946 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1527-3350 (Electronic) Linking ISSN: 02709139 NLM ISO Abbreviation: Hepatology Subsets: MEDLINE
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Dongelmans E;Erler N;Adam R;Nadalin S;Karam V;Yilmaz S;Kelly C;Pirenne J;Ac...
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Publisher: Wolters Kluwer Health, Inc Country of Publication: United States NLM ID: 8302946 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1527-3350 (Electronic) Linking ISSN: 02709139 NLM ISO Abbreviation: Hepatology Subsets: MEDLINE
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Background and Aims: Management of Budd-Chiari syndrome (BCS) has improved over the la...
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Recent outcomes of liver transplantation for Budd-Chiari syndrome: A study of the European Liver Transplant Registry (ELTR) and affiliated centers.
Publisher: Wolters Kluwer Health, Inc Country of Publication: United States NLM ID: 8302946 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1527-3350 (Electronic) Linking ISSN: 02709139 NLM ISO Abbreviation: Hepatology Subsets: MEDLINE
Background and Aims: Management of Budd-Chiari syndrome (BCS) has improved over the last decades. The main aim was to evaluate the contemporary post-liver transplantant (post-LT) outcomes in Europe.
Approach and Results: Data from all patients who underwent transplantation from 1976 to 2020 was obtained from the European Liver Transplant Registry (ELTR). Patients < 16 years with secondary BCS or HCC were excluded. Patient survival (PS) and graft survival (GS) before and after 2000 were compared. Multivariate Cox regression analysis identified predictors of PS and GS after 2000. Supplemental data was requested from all ELTR-affiliated centers and received from 44. In all, 808 patients underwent transplantation between 2000 and 2020. One-, 5- and 10-year PS was 84%, 77%, and 68%, and GS was 79%, 70%, and 62%, respectively. Both significantly improved compared to outcomes before 2000 ( p < 0.001). Median follow-up was 50 months and retransplantation rate was 12%. Recipient age (aHR:1.04,95%CI:1.02-1.06) and MELD score (aHR:1.04,95%CI:1.01-1.06), especially above 30, were associated with worse PS, while male sex had better outcomes (aHR:0.63,95%CI:0.41-0.96). Donor age was associated with worse PS (aHR:1.01,95%CI:1.00-1.03) and GS (aHR:1.02,95%CI:1.01-1.03). In 353 patients (44%) with supplemental data, 33% had myeloproliferative neoplasm, 20% underwent TIPS pre-LT, and 85% used anticoagulation post-LT. Post-LT anticoagulation was associated with improved PS (aHR:0.29,95%CI:0.16-0.54) and GS (aHR:0.48,95%CI:0.29-0.81). Hepatic artery thrombosis and portal vein thrombosis (PVT) occurred in 9% and 7%, while recurrent BCS was rare (3%).
Conclusions: LT for BCS results in excellent patient- and graft-survival. Older recipient or donor age and higher MELD are associated with poorer outcomes, while long-term anticoagulation improves both patient and graft outcomes.
(Copyright © 2024 American Association for the Study of Liver Diseases.)
Approach and Results: Data from all patients who underwent transplantation from 1976 to 2020 was obtained from the European Liver Transplant Registry (ELTR). Patients < 16 years with secondary BCS or HCC were excluded. Patient survival (PS) and graft survival (GS) before and after 2000 were compared. Multivariate Cox regression analysis identified predictors of PS and GS after 2000. Supplemental data was requested from all ELTR-affiliated centers and received from 44. In all, 808 patients underwent transplantation between 2000 and 2020. One-, 5- and 10-year PS was 84%, 77%, and 68%, and GS was 79%, 70%, and 62%, respectively. Both significantly improved compared to outcomes before 2000 ( p < 0.001). Median follow-up was 50 months and retransplantation rate was 12%. Recipient age (aHR:1.04,95%CI:1.02-1.06) and MELD score (aHR:1.04,95%CI:1.01-1.06), especially above 30, were associated with worse PS, while male sex had better outcomes (aHR:0.63,95%CI:0.41-0.96). Donor age was associated with worse PS (aHR:1.01,95%CI:1.00-1.03) and GS (aHR:1.02,95%CI:1.01-1.03). In 353 patients (44%) with supplemental data, 33% had myeloproliferative neoplasm, 20% underwent TIPS pre-LT, and 85% used anticoagulation post-LT. Post-LT anticoagulation was associated with improved PS (aHR:0.29,95%CI:0.16-0.54) and GS (aHR:0.48,95%CI:0.29-0.81). Hepatic artery thrombosis and portal vein thrombosis (PVT) occurred in 9% and 7%, while recurrent BCS was rare (3%).
Conclusions: LT for BCS results in excellent patient- and graft-survival. Older recipient or donor age and higher MELD are associated with poorer outcomes, while long-term anticoagulation improves both patient and graft outcomes.
(Copyright © 2024 American Association for the Study of Liver Diseases.)
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Tree-based algorithms for spatial modeling of soil particle distribution in arid and semi-arid region.
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Publisher: Springer Country of Publication: Netherlands NLM ID: 8508350 Publication Model: Electronic Cited Medium: Internet ISSN: 1573-2959 (Electronic) Linking ISSN: 01676369 NLM ISO Abbreviation: Environ Monit Assess Subsets: MEDLINE
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Abakay O;Kılıç M;Günal H;Kılıç OM
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Publisher: Springer Country of Publication: Netherlands NLM ID: 8508350 Publication Model: Electronic Cited Medium: Internet ISSN: 1573-2959 (Electronic) Linking ISSN: 01676369 NLM ISO Abbreviation: Environ Monit Assess Subsets: MEDLINE
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Accurate estimation of particle size distribution across a large area is crucial for p...
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Tree-based algorithms for spatial modeling of soil particle distribution in arid and semi-arid region.
Publisher: Springer Country of Publication: Netherlands NLM ID: 8508350 Publication Model: Electronic Cited Medium: Internet ISSN: 1573-2959 (Electronic) Linking ISSN: 01676369 NLM ISO Abbreviation: Environ Monit Assess Subsets: MEDLINE
Accurate estimation of particle size distribution across a large area is crucial for proper soil management and conservation, ensuring compatibility with capabilities and enabling better selection and adaptation of precision agricultural techniques. The study investigated the performance of tree-based models, ranging from simpler options like CART to sophisticated ones like XGBoost, in predicting soil texture over a wide geographic region. Models were constructed using remotely sensed plant and soil indexes as covariates. Variable selection employed the Boruta approach. Training and testing data for machine learning models consisted of particle size distribution results from 622 surface soil samples collected in southeastern Turkey. The XGBoost Clay model emerged as the most accurate predictor, with an R 2 value of 0.74. Its superiority was further underlined by a 21.36% relative improvement in XGBoost Clay RMSE compared to RF Clay and 44.5% compared to CART Clay . Similarly, the R 2 values for XGBoost Silt and XGBoost Sand models reached 0.71 and 0.75 in predicting sand and silt content, respectively. Among the considered covariates, the normalized ratio vegetation index and slope angle had the highest impact on clay content (21%), followed by topographic position index and simple ratio clay index (20%), while terrain ruggedness index had the least impact (18%). These results highlight the effectiveness of Boruta approach in selecting an adequate number of variables for digital mapping, suggesting its potential as a viable option in this field. Furthermore, the findings of this study suggest that remote sensing data can effectively contribute to digital soil mapping, with tree-based model development leading to improved prediction performance.
(© 2024. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)
(© 2024. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)
Subject terms:
Clay - Environmental Monitoring methods - Algorithms - Soil - SandContent provider:
MEDLINE
Evaluation of possible associated factors for early childhood caries: are preterm birth and birth weight related?
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Publisher: BioMed Central Country of Publication: England NLM ID: 101088684 Publication Model: Electronic Cited Medium: Internet ISSN: 1472-6831 (Electronic) Linking ISSN: 14726831 NLM ISO Abbreviation: BMC Oral Health Subsets: MEDLINE
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Selen MB;Demir P;Inceoglu F
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Publisher: BioMed Central Country of Publication: England NLM ID: 101088684 Publication Model: Electronic Cited Medium: Internet ISSN: 1472-6831 (Electronic) Linking ISSN: 14726831 NLM ISO Abbreviation: BMC Oral Health Subsets: MEDLINE
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Background: This study aimed to evaluate the oral and dental health of preschool child...
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Evaluation of possible associated factors for early childhood caries: are preterm birth and birth weight related?
Publisher: BioMed Central Country of Publication: England NLM ID: 101088684 Publication Model: Electronic Cited Medium: Internet ISSN: 1472-6831 (Electronic) Linking ISSN: 14726831 NLM ISO Abbreviation: BMC Oral Health Subsets: MEDLINE
Background: This study aimed to evaluate the oral and dental health of preschool children aged 12-71 months living in the Eastern Anatolia Region of Turkey, and to examine the effects of low birth weight (LBW) and preterm, early term and term birth on dental caries.
Methods: 475 participants were included in the study. Intraoral examinations were performed and evaluated for the presence of early childhood caries (ECC). These values are; Relationships such as age, gender, birth weight, week of birth, tooth brushing frequency, cariogenic nutrition, and parental education levels were examined. The obtained data were analyzed statistically (chi-square, t-test, artificial neural network (ANN)).
Results: Of the 475 participants, whose parents agreed to fill out the questionnaire, 250 were female and 225 were male. While the mean age was 49.78 ± 14.78 months for those with ECC, it was 38.93 ± 17.96 months for those without. Higher duration of breastfeeding (p = 0.04), education level of parents (p = 0.001), lower socioeconomic level (p = 0.001), and lower brushing frequency (p = 0.001) were also found to be significantly associated with ECC. ECC was seen in 90% of 77 children with a history of preterm birth. In LBW, this rate was 83%. According to the ANN result, in preterm birth; 12.9% affected ECC by LBW.
Conclusion: According to the results of our study, both LBW and preterm delivery were found to be associated with ECC and S-ECC (severe early childhood caries). An additional study on parents of preterm/LBW infants would be beneficial. In the early period, regular dental examination, implementation of preventive and preventive treatments, and nutrition education to parents can make a significant difference in the prevention of ECC.
(© 2024. The Author(s).)
Methods: 475 participants were included in the study. Intraoral examinations were performed and evaluated for the presence of early childhood caries (ECC). These values are; Relationships such as age, gender, birth weight, week of birth, tooth brushing frequency, cariogenic nutrition, and parental education levels were examined. The obtained data were analyzed statistically (chi-square, t-test, artificial neural network (ANN)).
Results: Of the 475 participants, whose parents agreed to fill out the questionnaire, 250 were female and 225 were male. While the mean age was 49.78 ± 14.78 months for those with ECC, it was 38.93 ± 17.96 months for those without. Higher duration of breastfeeding (p = 0.04), education level of parents (p = 0.001), lower socioeconomic level (p = 0.001), and lower brushing frequency (p = 0.001) were also found to be significantly associated with ECC. ECC was seen in 90% of 77 children with a history of preterm birth. In LBW, this rate was 83%. According to the ANN result, in preterm birth; 12.9% affected ECC by LBW.
Conclusion: According to the results of our study, both LBW and preterm delivery were found to be associated with ECC and S-ECC (severe early childhood caries). An additional study on parents of preterm/LBW infants would be beneficial. In the early period, regular dental examination, implementation of preventive and preventive treatments, and nutrition education to parents can make a significant difference in the prevention of ECC.
(© 2024. The Author(s).)
Subject terms:
Infant - Humans - Male - Child, Preschool - Infant, Newborn - Female - Birth Weight - Dental Caries Susceptibility - Breast Feeding - Risk Factors - Prevalence - Dental Caries epidemiology - Dental Caries etiology - Premature Birth epidemiology - Premature Birth etiologyContent provider:
MEDLINE
Cerebrotendinous Xanthomatosis patients with late diagnosed in single orthopedic clinic: two novel variants in the CYP27A1 gene.
Academic Journal
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Publisher: BioMed Central Country of Publication: England NLM ID: 101266602 Publication Model: Electronic Cited Medium: Internet ISSN: 1750-1172 (Electronic) Linking ISSN: 17501172 NLM ISO Abbreviation: Orphanet J Rare Dis Subsets: MEDLINE
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Köroğlu M;Karakaplan M;Gündüz E;Kesriklioğlu B;Ergen E;Aslantürk O;Özdemir ...
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Publisher: BioMed Central Country of Publication: England NLM ID: 101266602 Publication Model: Electronic Cited Medium: Internet ISSN: 1750-1172 (Electronic) Linking ISSN: 17501172 NLM ISO Abbreviation: Orphanet J Rare Dis Subsets: MEDLINE
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Background: Cerebrotendinous Xanthomatosis (CTX) is a rare autosomal recessive lipid s...
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Cerebrotendinous Xanthomatosis patients with late diagnosed in single orthopedic clinic: two novel variants in the CYP27A1 gene.
Publisher: BioMed Central Country of Publication: England NLM ID: 101266602 Publication Model: Electronic Cited Medium: Internet ISSN: 1750-1172 (Electronic) Linking ISSN: 17501172 NLM ISO Abbreviation: Orphanet J Rare Dis Subsets: MEDLINE
Background: Cerebrotendinous Xanthomatosis (CTX) is a rare autosomal recessive lipid storage disorder caused by loss of function variants in the CYP27A1 gene which encodes sterol 27-hydroxylase, on chromosome 2q35. Although the symptoms begin commonly in infancy, CTX diagnosis is often delayed. The aim of this study is to review the orthopedic findings of the disease by providing an overview of the clinical features of the disease. It is to raise awareness of this condition for which early diagnosis and treatment are important.
Methods: We retrospectively evaluated the clinical, laboratory, radiological, and genetic findings of eight patients from four families who were admitted to our Orthopedics and Traumatology Department between 2017 and 2022 due to bilateral Achilles tendon xanthomas, were found to have high cholestanol and CYP27A1 gene mutations.
Results: The mean age of patients was 37, and five of them were male. The mean age at the onset of symptoms was 9.25 years. The mean age of initial diagnosis was 33.75 years. Between symptom onset and clinical diagnosis, an average delay of 24.5 years was observed. All patients had bilateral Achilles tendon xanthoma. Notably, a novel variant (c.670_671delAA) in CYP27A1 gene was identified in three patients who also presented with peripheral neuropathy and bilateral pes cavus. One patient had osteoporosis and four patients had osteopenia. Five patients had a history of bilateral cataracts. Furthermore, three of the patients had early-onset chronic diarrhea and three of the patients had ataxia. Two of the patients had epilepsy and seven of the patients had behavior-personality disorder. All patients had low intelligence, but none of them had cardiac disease.
Conclusion: We present the diagnostic process and clinical features which the largest CTX case series ever reported from single orthopedic clinic. We suggest that patients with normal cholesterol levels presenting with xanthoma being genetically analyzed by testing at their serum cholestanol level, and that all siblings of patients diagnosed with CTX be examined.
(© 2024. The Author(s).)
Methods: We retrospectively evaluated the clinical, laboratory, radiological, and genetic findings of eight patients from four families who were admitted to our Orthopedics and Traumatology Department between 2017 and 2022 due to bilateral Achilles tendon xanthomas, were found to have high cholestanol and CYP27A1 gene mutations.
Results: The mean age of patients was 37, and five of them were male. The mean age at the onset of symptoms was 9.25 years. The mean age of initial diagnosis was 33.75 years. Between symptom onset and clinical diagnosis, an average delay of 24.5 years was observed. All patients had bilateral Achilles tendon xanthoma. Notably, a novel variant (c.670_671delAA) in CYP27A1 gene was identified in three patients who also presented with peripheral neuropathy and bilateral pes cavus. One patient had osteoporosis and four patients had osteopenia. Five patients had a history of bilateral cataracts. Furthermore, three of the patients had early-onset chronic diarrhea and three of the patients had ataxia. Two of the patients had epilepsy and seven of the patients had behavior-personality disorder. All patients had low intelligence, but none of them had cardiac disease.
Conclusion: We present the diagnostic process and clinical features which the largest CTX case series ever reported from single orthopedic clinic. We suggest that patients with normal cholesterol levels presenting with xanthoma being genetically analyzed by testing at their serum cholestanol level, and that all siblings of patients diagnosed with CTX be examined.
(© 2024. The Author(s).)
Subject terms:
Adult - Child - Female - Humans - Male - Cholestanol therapeutic use - Retrospective Studies - Xanthomatosis genetics - Cholestanetriol 26-Monooxygenase genetics - Xanthomatosis, Cerebrotendinous diagnosis - Xanthomatosis, Cerebrotendinous geneticsContent provider:
MEDLINE
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Augmentation with a non-vascularized autologous fibular graft for the management of Cierny-Mader type IV chronic femoral osteomyelitis: a salvage procedure.
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Publisher: Springer Verlag Country of Publication: Germany NLM ID: 7705431 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-5195 (Electronic) Linking ISSN: 03412695 NLM ISO Abbreviation: Int Orthop Subsets: MEDLINE
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Bas A;Balci HI;Kocaoglu M;Demirel M;Kochai A
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Publisher: Springer Verlag Country of Publication: Germany NLM ID: 7705431 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-5195 (Electronic) Linking ISSN: 03412695 NLM ISO Abbreviation: Int Orthop Subsets: MEDLINE
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Purpose: The study aimed to evaluate preliminary clinical and radiographic results of ...
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Augmentation with a non-vascularized autologous fibular graft for the management of Cierny-Mader type IV chronic femoral osteomyelitis: a salvage procedure.
Publisher: Springer Verlag Country of Publication: Germany NLM ID: 7705431 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-5195 (Electronic) Linking ISSN: 03412695 NLM ISO Abbreviation: Int Orthop Subsets: MEDLINE
Purpose: The study aimed to evaluate preliminary clinical and radiographic results of patients with Cierny-Mader type IV chronic femoral osteomyelitis and augmented with a non-vascularized fibular autograft as a salvage procedure because of the poorly regenerated new bone after bone transport over an intramedullary nail (BTON).
Methods: Patients diagnosed with CM type IV chronic femoral bone infection and treated with BTON procedure between 2003 and 2020 were retrospectively reviewed. Seven patients were included in the study whose distraction gap was poorly regenerated and then augmented with a non-vascularized fibular autograft. A three-stage treatment was administered. First, the infection was eradicated. Second, BTON was performed. Third, the poorly regenerated distraction gap was augmented with a fibular autograft before removing the external fixator (EF). Clinical and radiological results were evaluated based on the criteria described by Paley-Maar and Li classification.
Results: The mean patient age was 52 years. The mean treatment time was 24.8 months, with a mean femoral lengthening of 12.6 cm. The mean EF and bone healing indexes were 0.57 months/cm and 0.8 months/cm, respectively. The mean length of the fibular graft was 13 cm. The bone healing of new bones was achieved in all patients with good quality after grafting. Functional scores were excellent in four patients. No patients experienced any sequelae.
Conclusions: Non-vascularized fibular autograft augmentation may be an effective salvage procedure for poorly regenerated new bone after BTON to manage chronic femoral bone infection.
(© 2023. The Author(s) under exclusive licence to SICOT aisbl.)
Methods: Patients diagnosed with CM type IV chronic femoral bone infection and treated with BTON procedure between 2003 and 2020 were retrospectively reviewed. Seven patients were included in the study whose distraction gap was poorly regenerated and then augmented with a non-vascularized fibular autograft. A three-stage treatment was administered. First, the infection was eradicated. Second, BTON was performed. Third, the poorly regenerated distraction gap was augmented with a fibular autograft before removing the external fixator (EF). Clinical and radiological results were evaluated based on the criteria described by Paley-Maar and Li classification.
Results: The mean patient age was 52 years. The mean treatment time was 24.8 months, with a mean femoral lengthening of 12.6 cm. The mean EF and bone healing indexes were 0.57 months/cm and 0.8 months/cm, respectively. The mean length of the fibular graft was 13 cm. The bone healing of new bones was achieved in all patients with good quality after grafting. Functional scores were excellent in four patients. No patients experienced any sequelae.
Conclusions: Non-vascularized fibular autograft augmentation may be an effective salvage procedure for poorly regenerated new bone after BTON to manage chronic femoral bone infection.
(© 2023. The Author(s) under exclusive licence to SICOT aisbl.)
Subject terms:
Humans - Middle Aged - Autografts - Treatment Outcome - Retrospective Studies - Fibula transplantation - Bone Transplantation methods - Femur surgery - Osteomyelitis surgeryContent provider:
MEDLINE
Investigation of the miRNA levels changes to acceptable daily intake dose pesticide mixture exposure on rat mesentery and pancreas.
Academic Journal
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Publisher: Elsevier Science Ltd Country of Publication: England NLM ID: 0320657 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-1298 (Electronic) Linking ISSN: 00456535 NLM ISO Abbreviation: Chemosphere Subsets: MEDLINE
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Sevim C;Tsatsakis A;Taghizadehghalehjoughi A;Ozkaraca M;Kara M;Genc S;Mendi...
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Publisher: Elsevier Science Ltd Country of Publication: England NLM ID: 0320657 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-1298 (Electronic) Linking ISSN: 00456535 NLM ISO Abbreviation: Chemosphere Subsets: MEDLINE
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Consumers are constantly exposed to a variety of chemical mixtures as part of their ev...
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Investigation of the miRNA levels changes to acceptable daily intake dose pesticide mixture exposure on rat mesentery and pancreas.
Publisher: Elsevier Science Ltd Country of Publication: England NLM ID: 0320657 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-1298 (Electronic) Linking ISSN: 00456535 NLM ISO Abbreviation: Chemosphere Subsets: MEDLINE
Consumers are constantly exposed to a variety of chemical mixtures as part of their everyday activities and lifestyle. Food, water and commercial products are only some examples of the possible ways people get exposed to these mixtures. However, following federal and local guidelines for risk assessment related to chemical exposure, risk analysis focuses on a single substance exposure scenario and not on a mixture, as in real life. Realizing the pronounced gap of this methodology, the real-life risk simulation scenario approach tries to address this problem by investigating the possible effect of long-term exposure to chemical mixtures closely resembling the actual circumstances of modern life. As part of this effort, this study aimed to identify the cumulative effects of pesticides belonging to different classes and commonly used commercial products on long-term exposure with realistic doses. Sprague Dawley rats were given a pesticide mix of active ingredients and formulation chemicals in a daily acceptable dose (ADI) and 10xADI for 90 days. Following thorough everyday documentation of possible side-effects, after 90 days all animals were sacrificed and their organs were examined. Exposure to pesticides particularly affects the miRNA levels at that point will provide us with more information about whether they can be potential biomarkers.
Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests:Cigdem Sevim reports financial support was provided by Kastamonu University. Cigdem Sevim reports a relationship with Kastamonu University that includes: funding grants.
(Copyright © 2023 The Authors. Published by Elsevier Ltd.. All rights reserved.)
Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests:Cigdem Sevim reports financial support was provided by Kastamonu University. Cigdem Sevim reports a relationship with Kastamonu University that includes: funding grants.
(Copyright © 2023 The Authors. Published by Elsevier Ltd.. All rights reserved.)
Subject terms:
Humans - Rats - Animals - No-Observed-Adverse-Effect Level - Rats, Sprague-Dawley - Pancreas - Mesentery - Pesticides toxicity - MicroRNAsContent provider:
MEDLINE
Investigation of the Relationship Between Laparoscopic Sleeve Gastrectomy and Gastroesophageal Reflux Disease Using 24-hour Multichannel Intraluminal Impedance With pH Testing According to Current Consensus.
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Publisher: Lippincott Williams & Wilkins Country of Publication: United States NLM ID: 100888751 Publication Model: Electronic Cited Medium: Internet ISSN: 1534-4908 (Electronic) Linking ISSN: 15304515 NLM ISO Abbreviation: Surg Laparosc Endosc Percutan Tech Subsets: MEDLINE
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Teke E;Esen Bulut N;Onur E;Güneş Y;Fersahoglu MM;Ergin A;Taşdelen İ;Köroğlu...
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Publisher: Lippincott Williams & Wilkins Country of Publication: United States NLM ID: 100888751 Publication Model: Electronic Cited Medium: Internet ISSN: 1534-4908 (Electronic) Linking ISSN: 15304515 NLM ISO Abbreviation: Surg Laparosc Endosc Percutan Tech Subsets: MEDLINE
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Background: Laparoscopic sleeve gastrectomy (LSG) is a popular weight loss procedure w...
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Investigation of the Relationship Between Laparoscopic Sleeve Gastrectomy and Gastroesophageal Reflux Disease Using 24-hour Multichannel Intraluminal Impedance With pH Testing According to Current Consensus.
Publisher: Lippincott Williams & Wilkins Country of Publication: United States NLM ID: 100888751 Publication Model: Electronic Cited Medium: Internet ISSN: 1534-4908 (Electronic) Linking ISSN: 15304515 NLM ISO Abbreviation: Surg Laparosc Endosc Percutan Tech Subsets: MEDLINE
Background: Laparoscopic sleeve gastrectomy (LSG) is a popular weight loss procedure with potential effects on gastroesophageal reflux disease (GERD). However, research on the association between LSG and GERD using objective evaluation criteria, such as multichannel intraluminal impedance combined with pH testing (MII-pH), is limited. This study aimed to investigate the impact of LSG on GERD using MII-pH and current consensus guidelines.
Materials and Methods: It was conducted as a prospective clinical study on 33 patients who underwent LSG between January 2022 and August 2022. MII-pH and high-resolution manometry were performed preoperatively and 3 to 6 months postoperatively. GERD diagnosis was based on MII-pH results using the Lyon and Update Porto consensus guidelines.
Results: Postoperative MII-pH analysis revealed a significant increase in acid reflux time, acid exposure time, reflux index, esophageal clearance, total reflux time, and longest reflux period. Weak acid reflux episodes decreased, while Demeester score and alkaline reflux showed nonsignificant increases. Pathologic reflux significantly increased postoperatively based on MII-pH diagnosis. High-resolution manometry showed a significant increase in unsuccessful motility.
Conclusion: Although the Demeester score calculation consists of 6 metrics, including acid exposure time, the acid exposure time is more specific in detecting pathologic reflux. Pathologic GERD increases significantly with LSG in the early period. Therefore, preoperative and postoperative endoscopy and MII-pH can provide valuable information regarding the need for closer follow-up after LSG.
Competing Interests: The authors declare no conflicts of interest.
(Copyright © 2023 Wolters Kluwer Health, Inc. All rights reserved.)
Materials and Methods: It was conducted as a prospective clinical study on 33 patients who underwent LSG between January 2022 and August 2022. MII-pH and high-resolution manometry were performed preoperatively and 3 to 6 months postoperatively. GERD diagnosis was based on MII-pH results using the Lyon and Update Porto consensus guidelines.
Results: Postoperative MII-pH analysis revealed a significant increase in acid reflux time, acid exposure time, reflux index, esophageal clearance, total reflux time, and longest reflux period. Weak acid reflux episodes decreased, while Demeester score and alkaline reflux showed nonsignificant increases. Pathologic reflux significantly increased postoperatively based on MII-pH diagnosis. High-resolution manometry showed a significant increase in unsuccessful motility.
Conclusion: Although the Demeester score calculation consists of 6 metrics, including acid exposure time, the acid exposure time is more specific in detecting pathologic reflux. Pathologic GERD increases significantly with LSG in the early period. Therefore, preoperative and postoperative endoscopy and MII-pH can provide valuable information regarding the need for closer follow-up after LSG.
Competing Interests: The authors declare no conflicts of interest.
(Copyright © 2023 Wolters Kluwer Health, Inc. All rights reserved.)
